M17 Medium is a well buffered enriched medium for bacterial growth. The medium is optimized for isolating lactic Streptococci. Casein hydrolysate, Beef extract and Soya peptone supply carbon sources, nitrogen, vitamins and minerals essential for vigorous growth. Yeast extract supplies B-complex vitamins which stimulate bacteria growth. The medium is buffered by Disodium-β-glycerophosphate which prevents a drop in pH by the production of organic acids, such as lactic acid, during fermentation.

Ascorbic acid and Magnesium sulphate are added to stimulate growth.

Suspend 36.15 grams of powdered medium in 1 litre distilled water

Store dry at room temperature.