Phospho-MAPKAPK-2/MK2-T334 Polyclonal Antibody

Catalogue Numbers:BS74143-50, BS74143-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49137

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC,1:50 - 1:100

Background: This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded Mouse kidney using Phospho-MAPKAPK-2/MK2-T334 antibody at dilution of 1:200.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Rat heart using Phospho-MAPKAPK-2/MK2-T334 antibody at dilution of 1:200.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Human colon cancer using Phospho-MAPKAPK-2/MK2-T334 antibody at dilution of 1:200.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Human lung cancer using Phospho-MAPKAPK-2/MK2-T334 antibody at dilution of 1:200.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Alternative Name: MAPKAPK2;MAPKAP-K2;MK-2;MK2

Immunogen: A synthetic phosphorylated peptide around T334 of human MAPKAPK2(NP_116584.2?).

Conjugate: Unconjugated

Modification: Phosphorylated