Recombinant Human Carbonic Anhydrase 1/CA1 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032156-10, PKSH032156-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Carbonic Anhydrase 1; CA1

Target Symptom: Carbonic Anhydrase 1; Carbonate Dehydratase I; Carbonic Anhydrase B; CAB; Carbonic Anhydrase I; CA-I; CA1

Research Area: Cell biology; Cardiovascular

Target Species: Human

Expression Host: E.coli

Application: Enzyme

Fusion Tag: C-6His

UNIProt ID: P00915

Accession: P00915

Background: Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.

Activity: Measured by its esterase activity. The specific activity is 78.1 pmol/min/µg.

Sequence: Ala2-Phe261

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 29.9 kDa

Observed MW: 30 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only